Objective: To investigate the clinical application and outcomes of microdissection testicular sperm extraction (micro-TESE) in patients with nonmosaic Klinefelter syndrome (KS).
Methods: A total of 143 nonmosaic KS patients underwent micro-TESE in the Center of Reproductive Medicine of Peking University Third Hospital between July 2012 and August 2016. We analyzed their clinical and follow-up data and evaluated the outcomes.
Results: Spermatozoa were successfully retrieved from the testicular tissue in 44.76% (64/143) of the patients, 84.4% (54/64) by unilateral and 15.6% (10/64) by bilateral micro-TESE. Seventy-five of the KS patients were followed up in the years of 2014 and 2015. Of the 34 patients with successful sperm retrieval, 73.52% (25/34) achieved clinical pregnancy and 8 boys and 8 girls were already born in 14 of the 25 cases.
Conclusions: The micro-TESE is a useful method for sperm retrieval in nonmosaic KS patients, with high rates of sperm retrieval, clinical pregnancy, and birth of biological offspring.
目的： 探讨睾丸显微切开取精术在非嵌合型Klinefelter综合征患者中的临床应用及其结局。方法： 总结从2012年7月至2016年8月在我院生殖医学中心行睾丸显微切开取精术的143例非嵌合型Klinefelter综合征患者的临床资料并随访其治疗结局。结果： 143例非嵌合型Klinefelter综合征患者中有64例成功获取精子，精子获得率为44.76%（64/143）；其中顺利获得精子的64例患者中，有84.4%（54/64）患者仅行一侧睾丸显微切开取精即成功获取足够精子，15.6%（10/64）患者需行双侧睾丸显微切开取精获得精子。随访75例患者，成功获取精子的34例患者中，临床妊娠率为73.52%（25/34），已有14例患者妻子成功分娩8个男孩与8个女孩，目前有4例患者妻子正常临床妊娠中。结论： 睾丸显微切开取精术是使非嵌合型Klinefelter综合征患者成功获得自己遗传学子代的有效方法，有较高的精子获得率和临床受孕率。.
Keywords: Klinefelter syndrome; non-obstructive azoospermia; pregnancy rate; sperm retrieval; microdissection testicular sperm extraction.