Establishment of a spontaneously transformed cell line (JU-PI) from a myxoinflammatory fibroblastic sarcoma

Tumour Biol. 2018 May;40(5):1010428318777936. doi: 10.1177/1010428318777936.

Abstract

Myxoinflammatory fibroblastic sarcoma is a soft-tissue neoplasm most frequently found in the distal extremities of middle-aged adults. Most myxoinflammatory fibroblastic sarcoma are low-grade tumors with propensity for local recurrence after incomplete removal. We report a myxoinflammatory fibroblastic sarcoma which developed in the foot of a 41-year-old male and showed an exceptionally aggressive course with metastatic spread and fatal outcome within 16 months. We managed to establish a spontaneously transformed continuous cell line, called JU-PI, from a metastatic lesion. The JU-PI cells have a sub-tetraploid karyotype including the 1;10 chromosomal translocation and amplification of the proximal end of 3p; these features are considered genetic signatures of myxoinflammatory fibroblastic sarcoma. Both the primary tumor and the JU-PI cells showed nuclear expression of the TFE3 transcription factor but TFE3-activating chromosomal rearrangements were not found. To our knowledge, JU-PI is the first established myxoinflammatory fibroblastic sarcoma cell line. JU-PI cells offer a tool for investigating the molecular oncology of myxoinflammatory fibroblastic sarcoma.

Keywords: 3p11-3p12.1 amplification; Sarcoma cell line; TFE3; myxoinflammatory fibroblastic sarcoma.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
  • Cell Line, Transformed
  • Cell Proliferation
  • Dacarbazine / therapeutic use
  • Doxorubicin / therapeutic use
  • Fibrosarcoma* / drug therapy
  • Fibrosarcoma* / genetics
  • Fibrosarcoma* / mortality
  • Humans
  • Ifosfamide / therapeutic use
  • Karyotype
  • Male
  • Mesna / therapeutic use
  • Neoplasm Recurrence, Local / mortality
  • Neoplasm Recurrence, Local / pathology
  • Primary Cell Culture / methods*
  • Soft Tissue Neoplasms* / drug therapy
  • Soft Tissue Neoplasms* / genetics
  • Soft Tissue Neoplasms* / mortality
  • Translocation, Genetic / genetics
  • Vincristine / therapeutic use

Substances

  • Vincristine
  • Dacarbazine
  • Doxorubicin
  • Mesna
  • Ifosfamide

Supplementary concepts

  • IVADIC regimen