Abstract
The first systematic investigation of the effect of high mannose, hybrid, and bi- and tri-antennary complex type glycans on the effector functions of antibodies was achieved by the discovery of novel Endo-S2 mutants generated by site-directed mutagenesis as glycosynthases with broad substrate specificity.
MeSH terms
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Antibodies / chemistry*
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Antibodies / metabolism
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Glycoside Hydrolases / genetics
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Glycosylation
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Glycosyltransferases / chemistry*
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Glycosyltransferases / genetics
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Mutagenesis, Site-Directed
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Polysaccharides / chemistry*
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Protein Engineering
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Receptors, IgG / metabolism
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Streptococcus pyogenes / enzymology
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Structure-Activity Relationship
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Substrate Specificity
Substances
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Antibodies
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Polysaccharides
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Receptors, IgG
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Glycosyltransferases
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Glycoside Hydrolases