Improved outcomes of islet autotransplant after total pancreatectomy by combined blockade of IL-1β and TNFα

Am J Transplant. 2018 Sep;18(9):2322-2329. doi: 10.1111/ajt.14961. Epub 2018 Jun 25.

Abstract

The efficacy of islet transplant is compromised by a significant loss of islet mass posttransplant due to an innate inflammatory reaction. We report the use of a combination of etanercept and anakinra (ANA+ETA) to block inflammatory islet damage in 100 patients undergoing total pancreatectomy with islet autotransplant. The patients were divided into 3 groups: no treatment (control [CTL]), etanercept alone (ETA), or a combination of etanercept and anakinra (ANA+ETA). Peritransplant serum samples were analyzed for protein markers of islet damage and for inflammatory cytokines. Graft function was assessed by fasting blood glucose, basal C-peptide, secretory unit of islet transplant objects (SUITO) index, and hemoglobin A1c . Administration of both antiinflammatory drugs was well tolerated without any major adverse events. Reductions in interleukin-6, interleukin-8, and monocyte chemoattractant protein 1 were observed in patients receiving ANA+ETA compared with the CTL group, while also showing a modest improvement in islet function as assessed by basal C-peptide, glucose, hemoglobin A1c , and SUITO index but without differences in insulin dose. These results suggest that double cytokine blockade (ANA+ETA) reduces peritransplant islet damage due to nonspecific inflammation and may represent a promising strategy to improve islet engraftment, leading to better transplant outcomes.

Keywords: autotransplantation; clinical research/practice; innate immunity; islet transplantation; islets of Langerhans; translational research/science.

MeSH terms

  • Adult
  • Antirheumatic Agents / pharmacology
  • Autografts
  • Diabetes Mellitus, Type 1 / therapy*
  • Drug Therapy, Combination
  • Etanercept / pharmacology
  • Female
  • Follow-Up Studies
  • Graft Rejection / prevention & control*
  • Graft Survival*
  • Humans
  • Immunosuppressive Agents / pharmacology
  • Insulin Secretion
  • Interleukin 1 Receptor Antagonist Protein / pharmacology
  • Interleukin-1beta / antagonists & inhibitors*
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / metabolism
  • Islets of Langerhans Transplantation / methods*
  • Male
  • Pancreatectomy
  • Prognosis
  • Retrospective Studies
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors*

Substances

  • Antirheumatic Agents
  • Immunosuppressive Agents
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1beta
  • Tumor Necrosis Factor-alpha
  • Etanercept