Fusarium oxysporum (Fo) is an important genus of filamentous fungi that causes many devastating diseases of agronomical plants and some opportunistic diseases of humans. Previous studies have indicated that mutations in myosin5 acquired resistance to phenamacril in Fusarium graminearum (Fg). Here, we need to determine the residues of FoMyo5 involved in the natural resistance of plant pathogenic Fo strains. Six kinds of Fo reference strains from different hosts were studied. Fungicide susceptibility testing showed that these Fo strains demonstrated different resistance or susceptibility to phenamacril, which is Fusarium-specific antifungal compound, compared with Fg species. When aligned these homologous myosin5 motor domains of these strains, we found that the substitutions (Val151 to Ala and Ser418 to Thr) in FoMyo5 cause natural resistance to phenamacril in the plant pathogenic Fo strains. And we confirmed this result by gene replacement strategy. Such a phenomenon impeded the practical development of this fungicide for controlling vascular wilt diseases.
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