Autoantibody against integrin αv β3 contributes to thrombocytopenia by blocking the migration and adhesion of megakaryocytes

J Thromb Haemost. 2018 Sep;16(9):1843-1856. doi: 10.1111/jth.14214. Epub 2018 Jul 20.

Abstract

Essentials The pathogenesis of immune thrombocytopenia (ITP) has not been fully clarified. We analyzed the role of anti-αvβ3 autoantibody in the pathogenesis of ITP in patients. Anti-αvβ3 autoantibody impeded megakaryocyte migration and adhesion to the vascular niche. Anti-αv β3 autoantibody potentially contributes to the pathogenesis of refractory ITP.

Summary: Background The pathogenesis of immune thrombocytopenia (ITP) has not been fully clarified. Anti-αvβ3 integrin autoantibody is detected in chronic ITP patients, but its contribution to ITP is still unclear. Objectives To clarify the potential role of anti-αvβ3 integrin autoantibody in chronic ITP and the related mechanism. Methods Relationship between levels of anti-αvβ3 autoantibody and platelets in chronic ITP patients was evaluated. The influence of anti-αvβ3 antibody on megakaryocyte (MK) survival, differentiation, migration and adhesion was assessed, and the associated signal pathways were investigated. Platelet recovery and MKs' distribution were observed in an ITP mouse model pretreated with different antibodies. Result In this study, we showed that the anti-αvβ3 autoantibody usually coexists with anti-αIIbβ3 autoantibody in chronic ITP patients, and patients with both autoantibodies have lower platelets. In in vitro studies, we showed that the anti-αvβ3 antibody had no significant effect on the survival and proliferation of MKs, whereas it decreased formations of proplatelet significantly. Anti-αvβ3 antibody impeded stromal cell derived facor-1 alpha (SDF-1α)- mediated migration and inhibited the phosphorylation of protein kinase B. Anti-αvβ3 antibody significantly inhibited MKs' adhesion to endothelial cells and Fibrogen. The phosphorylation of focal adhesion kinase and proto-oncogene tyrosine-protein kinase Src induced by adhesion was inhibited when MKs were pretreated with anti-αvβ3 antibody. In in vivo studies, we showed that injection with anti-αv antibody delayed platelet recovery in a mouse model of ITP. Conclusions These findings demonstrate that the autoantibody against integrin αv β3 may aggravate thrombocytopenia in ITP patients by impeding MK migration and adhesion to the vascular niche, which provides new insights into the pathogenesis of ITP.

Keywords: adhesion; anti-αvβ3 autoantibody; immune thrombocytopenic purpura; megakaryocytes; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Autoantibodies / immunology*
  • Autoantigens / immunology*
  • Cell Adhesion
  • Cell Movement
  • Cells, Cultured
  • Chemokine CXCL12 / metabolism
  • Endothelial Cells / metabolism
  • Female
  • Fetal Blood / cytology
  • Humans
  • Integrin alphaVbeta3 / immunology*
  • Male
  • Megakaryocytes / cytology
  • Megakaryocytes / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Middle Aged
  • Phosphorylation
  • Platelet Count
  • Platelet Membrane Glycoprotein IIb / immunology
  • Protein Kinases / metabolism
  • Protein Processing, Post-Translational
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins c-akt / metabolism
  • Purpura, Thrombocytopenic, Idiopathic / blood
  • Purpura, Thrombocytopenic, Idiopathic / immunology*
  • Stromal Cells / metabolism
  • Thrombopoiesis
  • Young Adult

Substances

  • Autoantibodies
  • Autoantigens
  • Chemokine CXCL12
  • Integrin alphaVbeta3
  • MAS1 protein, human
  • Platelet Membrane Glycoprotein IIb
  • Proto-Oncogene Mas
  • Protein Kinases
  • Proto-Oncogene Proteins c-akt