Clinical, environmental, and behavioral characteristics associated with Cryptosporidium infection among children with moderate-to-severe diarrhea in rural western Kenya, 2008-2012: The Global Enteric Multicenter Study (GEMS)

PLoS Negl Trop Dis. 2018 Jul 12;12(7):e0006640. doi: 10.1371/journal.pntd.0006640. eCollection 2018 Jul.

Abstract

Background: Cryptosporidium is a leading cause of moderate-to-severe diarrhea (MSD) in young children in Africa. We examined factors associated with Cryptosporidium infection in MSD cases enrolled at the rural western Kenya Global Enteric Multicenter Study (GEMS) site from 2008-2012.

Methodology/principal findings: At health facility enrollment, stool samples were tested for enteric pathogens and data on clinical, environmental, and behavioral characteristics collected. Each child's health status was recorded at 60-day follow-up. Data were analyzed using logistic regression. Of the 1,778 children with MSD enrolled as cases in the GEMS-Kenya case-control study, 11% had Cryptosporidium detected in stool by enzyme immunoassay; in a genotyped subset, 81% were C. hominis. Among MSD cases, being an infant, having mucus in stool, and having prolonged/persistent duration diarrhea were associated with being Cryptosporidium-positive. Both boiling drinking water and using rainwater as the main drinking water source were protective factors for being Cryptosporidium-positive. At follow-up, Cryptosporidium-positive cases had increased odds of being stunted (adjusted odds ratio [aOR] = 1.65, 95% CI: 1.06-2.57), underweight (aOR = 2.08, 95% CI: 1.34-3.22), or wasted (aOR = 2.04, 95% CI: 1.21-3.43), and had significantly larger negative changes in height- and weight-for-age z-scores from enrollment.

Conclusions/significance: Cryptosporidium contributes significantly to diarrheal illness in young children in western Kenya. Advances in point of care detection, prevention/control approaches, effective water treatment technologies, and clinical management options for children with cryptosporidiosis are needed.

Publication types

  • Multicenter Study
  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Child, Preschool
  • Cryptosporidiosis / epidemiology
  • Cryptosporidiosis / parasitology*
  • Cryptosporidiosis / psychology
  • Cryptosporidium / genetics
  • Cryptosporidium / isolation & purification
  • Cryptosporidium / physiology*
  • Diarrhea / epidemiology
  • Diarrhea / parasitology*
  • Diarrhea / psychology
  • Feces / parasitology
  • Female
  • Humans
  • Infant
  • Kenya / epidemiology
  • Male
  • Prospective Studies
  • Rural Population

Grants and funding

The Bill & Melinda Gates Foundation (www.gatesfoundation.org) funded the Global Enteric Multicenter Study (GEMS) under grants #38874 (GEMS) and #OPP10333572 (GEMS1A) awarded to Principal Investigator MML. The U.S. Agency for International Development provided additional support for technical assistance through an Inter-Agency Agreement with the U.S. Centers for Disease Control and Prevention. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. All authors had full access to all the data.