Draft genome sequence of an NDM-1-, OXA-421- and AmpC-producing Acinetobacter pittii ST220 in Anhui Province, China

Yuchen Zhang; Shusheng Zhou

doi:10.1016/j.jgar.2018.07.008

Draft genome sequence of an NDM-1-, OXA-421- and AmpC-producing Acinetobacter pittii ST220 in Anhui Province, China

J Glob Antimicrob Resist. 2018 Sep:14:176-177. doi: 10.1016/j.jgar.2018.07.008. Epub 2018 Jul 20.

Authors

Yuchen Zhang¹, Shusheng Zhou²

Affiliations

¹ Department of Emergency Intensive Care Unit, Affiliated Provincial Hospital of Anhui Medical University, Anhui Medical University, No. 17 Lujiang Road, Hefei, Anhui, China.
² Department of Emergency Intensive Care Unit, Affiliated Provincial Hospital of Anhui Medical University, Anhui Medical University, No. 17 Lujiang Road, Hefei, Anhui, China; Department of Emergency Intensive Care Unit, First Affiliated Hospital of University of Science and Technology of China, University of Science and Technology of China, No. 17 Lujiang Road, Hefei, Anhui, China. Electronic address: zhouss108@163.com.

PMID: 30036696
DOI: 10.1016/j.jgar.2018.07.008

Abstract

Objectives: Acinetobacter pittii carrying the bla_NDM-1 gene is frequently reported in the world recently, however most of the bla_NDM-1 genes are located on plasmids. Here we report a multidrug-resistant (MDR) A. pittii isolated in China co-harbouring bla_NDM-1, bla_OXA-421 and bla_AmpC in the genome.

Methods: Bacterial genomic DNA was extracted using the cetyl trimethylammonium bromide (CTAB) method. Whole-genome sequencing of A. pittii was performed using an Illumina MiSeq system (2×251bp) in combination with PacBio single-molecule real-time (SMRT) sequencing. De novo genome assembly was performed using SPAdes v.3.9.0, A5-miseq v.20150522 and Canu v.1.4, respectively. The genome sequence was analysed by bioinformatics methods.

Results: The 4211131-bp genome with 38.99% G+C content displayed several resistance genes, including bla_NDM-1, bla_OXA-421 and bla_AmpC. Meanwhile, 4426 protein-coding sequences were predicted within the genome.

Conclusion: The genome sequence reported here can be compared with the already published genomes of NDM-1-producing isolates. These data might facilitate further understanding of the specific genomic feature of MDR A. pittii in China.

Keywords: Acinetobacter pittii; Multidrug resistance; Whole-genome sequencing; bla(NDM-1); bla(OXA-421).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acinetobacter Infections / microbiology
Acinetobacter baumannii / drug effects*
Acinetobacter baumannii / genetics*
Adult
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bacterial Typing Techniques
China
Computational Biology
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics*
Female
Genome, Bacterial*
Humans
Microbial Sensitivity Tests
Multilocus Sequence Typing
Sequence Analysis, DNA
Sputum / microbiology
Whole Genome Sequencing
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial
AmpC beta-lactamases
beta-Lactamases
beta-lactamase NDM-1