Hepatitis B virus transcripts in a human hepatoma cell line, Hep 3B

Biochem Biophys Res Commun. 1986 Jul 16;138(1):131-8. doi: 10.1016/0006-291x(86)90256-1.

Abstract

Hep 3B, a human hepatoma cell line was examined for its RNA hybridizable to the hepatitis B virus sequence. Using probes that covered different regions of the hepatitis B virus genome, five species of RNA were observed of sizes 4.0, 3.3, 2.9, 2.6 and 2.2 kilobases. The RNAs covered surface antigen gene, pre-S and X regions. None of them had a core antigen sequence. RNA with a 4.0 kilobase size was the most abundant. Using S1 nuclease analysis, its 5' end of hepatitis B virus sequence was mapped at pre-S region and its 3' end of viral sequence was mapped at DR region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / genetics
  • Antigens, Surface / genetics
  • Carcinoma, Hepatocellular / genetics*
  • Cell Line
  • DNA Restriction Enzymes / metabolism
  • DNA, Neoplasm / analysis
  • DNA, Viral / analysis
  • Deoxyribonuclease BamHI
  • Deoxyribonuclease EcoRI
  • Deoxyribonuclease HindIII
  • Endonucleases / metabolism
  • Hepatitis B virus / genetics*
  • Humans
  • Liver Neoplasms / genetics*
  • Nucleic Acid Hybridization
  • RNA, Neoplasm / analysis
  • RNA, Viral / analysis
  • Single-Strand Specific DNA and RNA Endonucleases
  • Transcription, Genetic*

Substances

  • Antigens, Neoplasm
  • Antigens, Surface
  • DNA, Neoplasm
  • DNA, Viral
  • RNA, Neoplasm
  • RNA, Viral
  • Endonucleases
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI
  • Deoxyribonuclease EcoRI
  • Deoxyribonuclease HindIII
  • Single-Strand Specific DNA and RNA Endonucleases