Performance evaluation of a newly developed molecular assay for the accurate diagnosis of gastroenteritis associated with norovirus of genogroup II

Arch Virol. 2018 Dec;163(12):3377-3381. doi: 10.1007/s00705-018-4010-8. Epub 2018 Sep 6.

Abstract

The performance of a newly proposed fully automated cassette-based sample-to-results solution for norovirus (NoV) detection, InGenius Norovirus ELITe MGB®, was evaluated. A total of 120 selected archival stool samples from children hospitalized for acute gastroenteritis were used to compare the results to a reference real-time RT-PCR. The InGenius NoV assay showed optimal diagnostic accuracy (sensitivity, 100%; specificity, 95.7%) and was able to correctly detect the entire wide panel of epidemiologically relevant genotypes tested. These preliminary results suggest that the InGenius NoV assay can be recommended as a valuable method for accurate diagnosis of NoV GII infection in epidemic and sporadic gastroenteritis.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Caliciviridae Infections / diagnosis*
  • Caliciviridae Infections / virology
  • Child
  • Child, Preschool
  • Feces / virology
  • Female
  • Gastroenteritis / diagnosis*
  • Gastroenteritis / virology
  • Genotype
  • Humans
  • Infant
  • Male
  • Norovirus / classification
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction / instrumentation
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity