Comparative study of adjuvants for allergen-specific immunotherapy in a murine model

Immunotherapy. 2018 Oct;10(14):1219-1228. doi: 10.2217/imt-2018-0072. Epub 2018 Sep 24.

Abstract

Aim: To compare the immunological and clinical changes induced by allergen-specific immunotherapy (AIT) using different adjuvants.

Materials & methods: Olea europaea pollen-sensitized mice were treated with olea plus aluminum hydroxide, calcium phosphate, monophosphoryl lipid A (MPL) or immunostimulatory sequences (ISS).

Results: Aluminum hydroxide seems to drive initially to a Th2-type response. Bacteria-derived adjuvants (MPL and ISS) skew the immune response toward Th1 and Treg pathways. Specific-IgE production was lower after AIT with MPL and ISS. Moreover, IgG2a production significantly increased in ISS-treated mice. Bacteria-derived adjuvants also improved the Th1 cytokine response due to IFN-γ higher secretion. In addition, they improved bronchial hyper-reactivity and lung inflammation.

Conclusion: Bacteria-derived adjuvants may enhance the efficacy of AIT.

Keywords: adjuvants; allergen-specific immunotherapy; allergic asthma; aluminum hydroxide; calcium phosphate; immunostimulatory sequences; monophosphoryl lipid A; murine model.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / therapeutic use*
  • Allergens / immunology
  • Allergens / therapeutic use*
  • Aluminum Hydroxide / therapeutic use
  • Animals
  • Antigens, Plant / immunology
  • Calcium Phosphates / therapeutic use
  • Desensitization, Immunologic / methods*
  • Disease Models, Animal
  • Female
  • Humans
  • Hypersensitivity / immunology
  • Hypersensitivity / therapy*
  • Lipid A / analogs & derivatives
  • Lipid A / therapeutic use
  • Mice
  • Mice, Inbred BALB C
  • Olea / immunology
  • Plant Extracts / immunology
  • Plant Extracts / therapeutic use*
  • Pollen / immunology
  • Respiratory Function Tests

Substances

  • Adjuvants, Immunologic
  • Allergens
  • Antigens, Plant
  • Calcium Phosphates
  • Lipid A
  • Plant Extracts
  • Aluminum Hydroxide
  • calcium phosphate
  • monophosphoryl lipid A