VEGF-B is a potent antioxidant

Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10351-10356. doi: 10.1073/pnas.1801379115. Epub 2018 Sep 24.

Abstract

VEGF-B was discovered a long time ago. However, unlike VEGF-A, whose function has been extensively studied, the function of VEGF-B and the mechanisms involved still remain poorly understood. Notwithstanding, drugs that inhibit VEGF-B and other VEGF family members have been used to treat patients with neovascular diseases. It is therefore critical to have a better understanding of VEGF-B function and the underlying mechanisms. Here, using comprehensive methods and models, we have identified VEGF-B as a potent antioxidant. Loss of Vegf-b by gene deletion leads to retinal degeneration in mice, and treatment with VEGF-B rescues retinal cells from death in a retinitis pigmentosa model. Mechanistically, we demonstrate that VEGF-B up-regulates numerous key antioxidative genes, particularly, Gpx1 Loss of Gpx1 activity largely diminished the antioxidative effect of VEGF-B, demonstrating that Gpx1 is at least one of the critical downstream effectors of VEGF-B. In addition, we found that the antioxidant function of VEGF-B is mediated mainly by VEGFR1. Given that oxidative stress is a crucial factor in numerous human diseases, VEGF-B may have therapeutic value for the treatment of such diseases.

Keywords: Gpx1; VEGF-B; antioxidant; oxidative stress; retinal degeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Neutralizing / pharmacology
  • Antioxidants / metabolism*
  • Apoptosis / drug effects
  • Apoptosis / genetics
  • Disease Models, Animal
  • Gene Expression Regulation
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase GPX1
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Oxidative Stress
  • Retina / drug effects
  • Retina / pathology
  • Retinal Degeneration / drug therapy
  • Retinal Degeneration / genetics*
  • Retinitis Pigmentosa / genetics
  • Vascular Endothelial Growth Factor B / genetics
  • Vascular Endothelial Growth Factor B / metabolism*
  • Vascular Endothelial Growth Factor B / pharmacology
  • Vascular Endothelial Growth Factor Receptor-1 / genetics
  • Vascular Endothelial Growth Factor Receptor-1 / metabolism

Substances

  • Antibodies, Neutralizing
  • Antioxidants
  • Vascular Endothelial Growth Factor B
  • vascular endothelial growth factor B, mouse
  • Glutathione Peroxidase
  • Vascular Endothelial Growth Factor Receptor-1
  • Glutathione Peroxidase GPX1
  • Gpx1 protein, mouse