The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo

Lab Invest. 2019 Mar;99(3):399-410. doi: 10.1038/s41374-018-0116-6. Epub 2018 Oct 5.

Abstract

The dual specificity phosphatase slingshot homolog 1 (SSH1) contributes to actin remodeling by dephosphorylating and activating the actin-severing protein cofilin. The reorganization of the actin cytoskeleton has been implicated in chronic hypertension and the subsequent mechano-adaptive rearrangement of vessel wall components. Therefore, using a novel Ssh1-/- mouse model, we investigated the potential role of SSH1 in angiotensin II (Ang II)-induced hypertension, and vascular remodeling. We found that loss of SSH1 did not produce overt phenotypic changes and that baseline blood pressures as well as heart rates were comparable between Ssh1+/+ and Ssh1-/- mice. Although 14 days of Ang II treatment equally increased systolic blood pressure in both genotypes, histological assessment of aortic samples indicated that medial thickening was exacerbated by the loss of SSH1. Consequently, reverse-transcription quantitative PCR analysis of the transcripts from Ang II-infused animals confirmed increased aortic expression levels of fibronectin, and osteopontin in Ssh1-/- when compared to wild-type mice. Mechanistically, our data suggest that fibrosis in SSH1-deficient mice occurs by a process that involves aberrant responses to Ang II-induced TGFβ1. Taken together, our work indicates that Ang II-dependent fibrotic gene expression and vascular remodeling, but not the Ang II-induced pressor response, are modulated by SSH1-mediated signaling pathways and SSH1 activity is protective against Ang II-induced remodeling in the vasculature.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Angiotensin II / metabolism*
  • Animals
  • Aorta / metabolism
  • Aorta / pathology
  • Disease Models, Animal
  • Female
  • Fibrosis
  • Hypertension / etiology
  • Hypertension / metabolism
  • Hypertension / pathology
  • Hypertrophy
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phosphoprotein Phosphatases / deficiency
  • Phosphoprotein Phosphatases / genetics
  • Phosphoprotein Phosphatases / metabolism*
  • Transforming Growth Factor beta1 / metabolism
  • Vascular Remodeling / genetics
  • Vascular Remodeling / physiology*

Substances

  • Tgfb1 protein, mouse
  • Transforming Growth Factor beta1
  • Angiotensin II
  • Phosphoprotein Phosphatases
  • SSH1 protein, mouse