Evaluation of Peritumoral Fibrosis in Metastatic Colorectal Adenocarcinoma to the Liver Using Digital Image Analysis

Am J Clin Pathol. 2019 Jan 7;151(2):226-230. doi: 10.1093/ajcp/aqy134.

Abstract

Objectives: It is challenging to separate peritumoral fibrosis from fibrosis due to chronic liver disease in mass-directed liver biopsies. We evaluated the distance that peritumoral fibrosis extends from metastatic colorectal adenocarcinoma in liver.

Methods: Peritumoral and distant uninvolved liver trichrome stains from 25 cases were analyzed using digital image analysis. Fibrosis was quantitated at concentric intervals from each tumor and in uninvolved liver.

Results: There was a 3.9 fold (range 0.9-18.6) median increase in fibrosis in the first 0.5 mm of peritumoral liver compared to distant liver. Fibrosis levels returned to baseline at median 2.5 mm (interquartile range 1.5-5.0 mm) from tumor.

Conclusions: Fibrosis is markedly increased in peritumoral liver. Fibrosis levels returned to baseline by 5 mm from tumor in approximately 75% of cases. Pathologists should be cautious of fibrosis in mass-directed liver biopsies without at least 5 mm of liver tissue distal to the mass.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / diagnosis
  • Adenocarcinoma / secondary*
  • Adenocarcinoma / surgery
  • Adult
  • Aged
  • Azo Compounds
  • Colorectal Neoplasms / diagnosis
  • Colorectal Neoplasms / pathology*
  • Colorectal Neoplasms / surgery
  • Coloring Agents
  • Eosine Yellowish-(YS)
  • Female
  • Fibrosis / diagnosis
  • Fibrosis / pathology*
  • Humans
  • Image Processing, Computer-Assisted
  • Immunohistochemistry
  • Liver / pathology
  • Liver / surgery
  • Liver Neoplasms / diagnosis
  • Liver Neoplasms / secondary*
  • Liver Neoplasms / surgery
  • Male
  • Methyl Green
  • Middle Aged
  • Pathology, Surgical
  • Software

Substances

  • Azo Compounds
  • Coloring Agents
  • trichrome stain
  • Methyl Green
  • Eosine Yellowish-(YS)