Characterization of FOXO Acetylation

Methods Mol Biol. 2019:1890:77-90. doi: 10.1007/978-1-4939-8900-3_7.

Abstract

FOXO3 is a tumor suppressor that orchestrates the expression of genes that regulate cell cycle progression, apoptosis, metabolism, oxidative stress, and other important cellular processes. Its inactivation is closely associated with tumorigenesis and cancer progression. On the other hand, sirtuin proteins have been demonstrated to be able to deacetylate, thus causing FOXO3 inactivation at the posttranslational level. Therefore, targeting sirtuin proteins renders new avenues for breast cancer treatment. Here, we describe three procedures for studying FOXO3 posttranslational modifications controlled by sirtuin proteins in cancer cells.

Keywords: Acetylation; Deacetylation; FOXO3; SIRT1; SIRT2; Sirtuin.

MeSH terms

  • Acetylation
  • Blotting, Western
  • Cell Line
  • Cells, Cultured
  • Fluorescent Antibody Technique
  • Forkhead Transcription Factors / metabolism*
  • Gene Expression
  • Humans
  • Immunoprecipitation
  • Protein Processing, Post-Translational*
  • RNA, Small Interfering / genetics
  • Transfection

Substances

  • Forkhead Transcription Factors
  • RNA, Small Interfering