Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation

Chuanyin Wu; Yi Sui

doi:10.1007/978-1-4939-8778-8_7

Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation

Methods Mol Biol. 2019:1864:95-103. doi: 10.1007/978-1-4939-8778-8_7.

Authors

Chuanyin Wu¹, Yi Sui²

Affiliations

¹ The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China. wuchuanyin@caas.cn.
² The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

PMID: 30415331
DOI: 10.1007/978-1-4939-8778-8_7

Abstract

Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The protocol simplifies medium compositions and transformation steps and can be easily followed by a lab technician with little tissue culture experience. Using this protocol, we have transformed thousands of gene constructs in the past 10 years and edited hundreds of genes with the CRISPR-Cas9 system recently.

Keywords: Agrobacterium-mediated transformation; Callus induction; Plant regeneration; Rice; Selection of transformants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium / genetics*
Oryza / genetics*
Plant Shoots
Plants, Genetically Modified / genetics*
Seeds
Time Factors
Tissue Culture Techniques / instrumentation
Tissue Culture Techniques / methods
Transformation, Genetic*