On-chip Detection of Single Vesicle Release from Neuroblastoma Cells using Monolithic CMOS Bioelectronics

Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul:2018:5065-5068. doi: 10.1109/EMBC.2018.8513219.

Abstract

Neuroblastoma cells are often used as a cell model to study Parkinson's disease, which causes reduced dopamine release in substantia nigra, the midbrain that controls movements. In this paper, we developed a 1024-ch monolithic CMOS sensor array that has the spatiotemporal resolution as well as low-noise performance to monitor single vesicle release of dopamine from neuroblastoma cells. The CMOS device integrates 1024 on-chip electrodes with an individual size of $15 \mu \mathrm{m}\times 15 \mu \mathrm{m}$ and 1024 transimpedance amplifiers for each electrode, which are each capable of measuring sub-pA current. Thus, this device can be used to study the detailed molecular dynamics of dopamine secretion at single vesicle resolution.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amplifiers, Electronic
  • Dopamine
  • Electrodes
  • Humans
  • Neuroblastoma*
  • Substantia Nigra*

Substances

  • Dopamine