Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry

Monoclon Antib Immunodiagn Immunother. 2019 Apr;38(2):96-99. doi: 10.1089/mab.2018.0031. Epub 2018 Nov 20.

Abstract

Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG1, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.

Keywords: PDPN; PMab-44; goat podoplanin.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity / immunology*
  • CHO Cells
  • Cattle
  • Cricetinae
  • Cricetulus
  • Goats
  • Immunohistochemistry / methods*
  • Lung / immunology
  • Lung / metabolism*
  • Membrane Glycoproteins / immunology*

Substances

  • Antibodies, Monoclonal
  • Membrane Glycoproteins