Comparison of metalworking fluids biodegradation efficiency by autochthonous and environmental communities

Abstract

The aim of this study was to evaluate the biodegradation potential of microbiota isolated from different environmental niches towards different types of metalworking fluids (MWF). The first experimental stage was focused on the assessment of biochemical oxygen demand reduction efficiency of autochthonous and environmental microbial communities. Based on the obtained results, the following order describing the biodegradation potential of communities from the studied niches was established: petroleum contaminated soil > waste repository ≥ waste MWF tanks > pesticide-treated field > activated sludge > municipal sewage effluents. For comparative purposes, the most efficient community originating from petroleum contaminated soil (PCS1) was selected for further studies along with the most efficient community originating from a waste MWF tank (WMT1). The studied communities achieved 100% biodegradation efficiency of decanedioic and dodecanedioic acids as well as glycerine and polyethoxylated dodecanol. However, the PCS1 community was more versatile and displayed significantly higher biodegradation efficiency of mineral oil (80% compared to 50% in case of WMT1). Similarly, experiments using pristine and spent MWF solutions confirmed that the PCS1 community outperformed the WMT1 community during the biodegradation of MWF containing oil as the main component (COD reduction of 80, 60 and 30% in case of semi-synthetic MWF, soluble oil and spent MWF, respectively). Results of community dynamics assessment using quantitative real-time PCR after the biodegradation of different types of MWF confirmed that the PCS1 community was characterized by high genetic stability and allowed to indicate the potential 'key players'.

Keywords: Bacterial communities; Biodegradation; Community dynamics; Metalworking fluids.