Expression of tumor necrosis factor in vitro by human mononuclear phagocytes stimulated with whole Mycobacterium bovis BCG and mycobacterial antigens

S E Valone; E A Rich; R S Wallis; J J Ellner

doi:10.1128/iai.56.12.3313-3315.1988

Expression of tumor necrosis factor in vitro by human mononuclear phagocytes stimulated with whole Mycobacterium bovis BCG and mycobacterial antigens

Infect Immun. 1988 Dec;56(12):3313-5. doi: 10.1128/iai.56.12.3313-3315.1988.

Authors

S E Valone¹, E A Rich, R S Wallis, J J Ellner

Affiliation

¹ Department of Medicine, Case Western Reserve University, Cleveland, Ohio.

Abstract

Four mycobacterial preparations directly stimulated human blood monocytes and alveolar macrophages to produce tumor necrosis factor (TNF). Monoclonal antibody against TNF blocked (99%) TNF activity. Lipopolysaccharide was not responsible for the TNF activity generated; activity was unaffected in the presence of polymyxin B.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Antigens, Bacterial / immunology*
Bronchoalveolar Lavage Fluid
Humans
In Vitro Techniques
Leukocytes, Mononuclear / immunology*
Lipid A / antagonists & inhibitors
Macrophage Activation
Macrophages / immunology*
Mycobacterium bovis / immunology*
Polymyxin B / pharmacology
Time Factors
Tuberculin / immunology
Tumor Necrosis Factor-alpha / biosynthesis*

Substances

Antigens, Bacterial
Lipid A
Tuberculin
Tumor Necrosis Factor-alpha
Polymyxin B

Grants and funding

AI18471/AI/NIAID NIH HHS/United States