[Development and evaluation of an in-house database for quick identification of Burkholderia contaminans by MALDI-TOF MS]

Rev Argent Microbiol. 2019 Jul-Sep;51(3):255-258. doi: 10.1016/j.ram.2018.09.001. Epub 2018 Dec 15.
[Article in Spanish]

Abstract

MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in them, MALDI-TOF MS emerges as a strategic tool. The aim of this work was to develop an additional database with peptide spectra of reference isolates of B. contaminans. This database demonstrated to be successful for the identification of 97% of the isolates analyzed. Therefore, MALDI-TOF MS with the extended database was a useful tool for the identification and differentiation of other related species to B. contaminans.

Keywords: B. contaminans; EM MALDI-TOF; Identificación; Identification; MALDI-TOF MS.

Publication types

  • Evaluation Study

MeSH terms

  • Algorithms
  • Bacterial Proteins / analysis
  • Bacteriological Techniques*
  • Burkholderia / chemistry
  • Burkholderia / classification
  • Burkholderia / isolation & purification*
  • Burkholderia Infections / complications
  • Burkholderia Infections / microbiology
  • Cystic Fibrosis / complications
  • Cystic Fibrosis / microbiology
  • Databases, Factual*
  • Humans
  • Reproducibility of Results
  • Species Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Bacterial Proteins

Supplementary concepts

  • Burkholderia contaminans