RXXPEG motif of MERIT40 is required to maintain spindle structure and function through its interaction with Tankyrase1

Cell Biol Int. 2019 Feb;43(2):174-181. doi: 10.1002/cbin.11086.

Abstract

Deubiquitinase BRISC complex plays important role in the maintenance of spindle structure and function; however, the underlying mechanism remains largely undefined. Here we demonstrated that MERIT40, a core component of BRISC complex, directly interacts with the RXXPEG motif in the ARC-V domain of Tankyrase1(TNKS1). Mutation of the RXXPEG motif in the MERIT40 (R28A) disrupted its interaction with TNKS1. Consistent with these data, R28A mutant cells displayed multiple mitotic defects including aberrant spindle assembly and chromosome misalignment. These results support a critical role of RXXPEG motif of MERIT40 in BRISC-mediated regulation of TNKS1 function during spindle assembly.

Keywords: MERIT40; RXXPEG motif; TNKS1; genome stability.

MeSH terms

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing / chemistry
  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Amino Acid Motifs
  • Deubiquitinating Enzymes
  • HeLa Cells
  • Humans
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism
  • Mutagenesis, Site-Directed
  • Protein Binding
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Sequence Alignment
  • Spindle Apparatus / metabolism*
  • Tankyrases / chemistry
  • Tankyrases / metabolism*

Substances

  • 3' Untranslated Regions
  • Adaptor Proteins, Signal Transducing
  • BABAM1 protein, human
  • Membrane Proteins
  • RNA, Small Interfering
  • Tankyrases
  • TNKS protein, human
  • BRCC3 protein, human
  • Deubiquitinating Enzymes