Prevalence and antifungal susceptibility of Sporothrix species in Jiangxi, central China

Med Mycol. 2019 Nov 1;57(8):954-961. doi: 10.1093/mmy/myy163.

Abstract

Sporotrichosis is a subcutaneous mycosis caused by traumatic inoculation of pathogenic Sporothrix species. Until recently, Sporothrix globosa was considered as the unique Chinese species causing this disorder. In the present study, 33 clinical Sporothrix strains isolated from Jiangxi, China, were classified and antifungal susceptibility for each strain was determined. Thirteen S. globosa strains and 20 S. schenckii strains were identified by morphology and by multilocus analysis using rDNA ITS, CAL, and EF1α (i.e., internal transcribed spacer, calmodulin and elongation factor-1α). In vitro antifungal susceptibility testing of yeast phases indicated that itraconazole, terbinafine, and posaconazole were most effective against both species, followed by amphotericin B and voriconazole, while fluconazole, 5-fluorocytosine had low efficacy with high MICs. Co-occurrence of S. schenckii and S. globosa in central China may indicate different routes of transmission in this area.

Keywords: Sporothrix globosa; Sporothrix schenckii; China; antifungal susceptibility; molecular identification; sporotrichosis; yeast phase.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Antifungal Agents / pharmacology*
  • Child
  • Child, Preschool
  • China / epidemiology
  • DNA, Ribosomal Spacer / genetics
  • Female
  • Genotype
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Microscopy
  • Middle Aged
  • Multilocus Sequence Typing
  • Peptide Elongation Factor 1 / genetics
  • Prevalence
  • Sporothrix / classification*
  • Sporothrix / drug effects*
  • Sporothrix / isolation & purification
  • Sporotrichosis / epidemiology*
  • Sporotrichosis / microbiology*
  • Young Adult

Substances

  • Antifungal Agents
  • DNA, Ribosomal Spacer
  • Peptide Elongation Factor 1