A genetically encoded near-infrared fluorescent calcium ion indicator

Yong Qian; Kiryl D Piatkevich; Benedict Mc Larney; Ahmed S Abdelfattah; Sohum Mehta; Mitchell H Murdock; Sven Gottschalk; Rosana S Molina; Wei Zhang; Yingche Chen; Jiahui Wu; Mikhail Drobizhev; Thomas E Hughes; Jin Zhang; Eric R Schreiter; Shy Shoham; Daniel Razansky; Edward S Boyden; Robert E Campbell

doi:10.1038/s41592-018-0294-6

A genetically encoded near-infrared fluorescent calcium ion indicator

Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6. Epub 2019 Jan 21.

Authors

Yong Qian¹, Kiryl D Piatkevich², Benedict Mc Larney^{3

4}, Ahmed S Abdelfattah⁵, Sohum Mehta⁶, Mitchell H Murdock², Sven Gottschalk³, Rosana S Molina⁷, Wei Zhang¹, Yingche Chen¹, Jiahui Wu¹, Mikhail Drobizhev⁷, Thomas E Hughes⁷, Jin Zhang⁶, Eric R Schreiter⁵, Shy Shoham⁸, Daniel Razansky^{3

4

9

10}, Edward S Boyden², Robert E Campbell^{11

12}

Affiliations

¹ Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
² Media Lab and McGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
³ Institute for Biological and Medical Imaging, Helmholtz Center Munich, Neuherberg, Germany.
⁴ Faculty of Medicine, Technical University of Munich, Munich, Germany.
⁵ Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
⁶ Department of Pharmacology, University of California San Diego, La Jolla, CA, USA.
⁷ Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT, USA.
⁸ Departments of Ophthalmology and of Neuroscience and Physiology, New York University Langone Health, New York City, NY, USA.
⁹ Faculty of Medicine and Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
¹⁰ Department of Information Technology and Electrical Engineering and Institute for Biomedical Engineering, ETH Zurich, Zurich, Switzerland.
¹¹ Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. robert.e.campbell@ualberta.ca.
¹² Department of Chemistry, Graduate School of Science, The University of Tokyo, Tokyo, Japan. robert.e.campbell@ualberta.ca.

Abstract

We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca²⁺) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca²⁺ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca²⁺ imaging in combination with other optogenetic indicators and actuators.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Biliverdine / chemistry
Calcium / chemistry*
DNA / analysis
Escherichia coli / chemistry
Female
Fluorescence Resonance Energy Transfer
Fluorescent Dyes / chemistry*
Genetic Vectors
HeLa Cells
Hippocampus / chemistry
Humans
Ions
Male
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Neurons / chemistry
Optogenetics
Protein Domains
Spectroscopy, Near-Infrared / methods*

Substances

Fluorescent Dyes
Ions
DNA
Biliverdine
Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding