A metagenomic analysis of the modulatory effect of Cyclocarya paliurus flavonoids on the intestinal microbiome in a high-fat diet-induced obesity mouse model

J Sci Food Agric. 2019 Jun;99(8):3967-3975. doi: 10.1002/jsfa.9622. Epub 2019 Mar 5.

Abstract

Background: As a result of a low bioavailability, the majority of Cyclocarya paliurus flavonoids (CPF) remain in the large intestine where they accumulate to exert a modulatory effect on the intestinal micro-ecology. Therefore, the present study investigated the modulatory effect of CPF on intestinal microbiota.

Results: CPF dramatically ameliorated the obesity-induced gut dysbiosis. A significant decrease (P < 0.05) was observed in the ratio of Firmicutes/Bacteroidetes after CPF treatment for 8 weeks. Moreover, Kyoto Encyclopedia of Genes and Genomes pathways of biosynthesis of amino acids, the two-component system and ATP-binding cassette transporters enriched the most differentially expressed genes after CPF intervention.

Conclusion: The results of the present study indicate that CPF might have prebiotic-like activity and could be used as a functional food component with potential therapeutic utility to prevent obesity-related metabolic disorders by manipulating the gut flora and affecting certain metabolic pathways, thus contributing to the improvement of human health. © 2019 Society of Chemical Industry.

Keywords: Cyclocarya paliurus flavonoids; intestinal microbiome; metagenomics; modulatory effect; obesity.

MeSH terms

  • Adult
  • Animals
  • Bacteria / classification
  • Bacteria / drug effects
  • Bacteria / genetics*
  • Bacteria / isolation & purification
  • Diet, High-Fat / adverse effects
  • Disease Models, Animal
  • Dysbiosis / drug therapy
  • Dysbiosis / metabolism
  • Dysbiosis / microbiology
  • Female
  • Flavonoids / administration & dosage*
  • Flavonoids / analysis
  • Gastrointestinal Microbiome / drug effects*
  • Humans
  • Intestines / microbiology*
  • Juglandaceae / chemistry*
  • Male
  • Metagenomics
  • Mice
  • Mice, Inbred C57BL
  • Obesity / drug therapy*
  • Obesity / microbiology
  • Plant Extracts / administration & dosage*
  • Plant Extracts / analysis

Substances

  • Flavonoids
  • Plant Extracts