Tagging of a cryptic promoter that confers root-specific gus expression in Arabidopsis thaliana

P Mollier; B Hoffmann; M Orsel; G Pelletier

doi:10.1007/s002990000241

Tagging of a cryptic promoter that confers root-specific gus expression in Arabidopsis thaliana

Plant Cell Rep. 2000 Nov;19(11):1076-1083. doi: 10.1007/s002990000241.

Authors

P Mollier¹, B Hoffmann¹, M Orsel¹, G Pelletier¹

Affiliation

¹ Station de Génétique et d'Amélioration des Plantes, I.N.R.A, 78026 Versailles Cedex, France e-mail: mollier@versailles.inra.fr Fax: +33-1-30833319, , , , , , FR.

PMID: 30754773
DOI: 10.1007/s002990000241

Abstract

A 2.1-kb sequence was isolated by promoter trapping from an Arabidopsis thaliana transformant (T80) obtained by Agrobacterium-mediated T-DNA insertion. This sequence directed strong β-glucuronidase (GUS) expression specifically in roots. The promoter-gus fusion was used to transform other A. thaliana plants. Most of the transformants obtained exhibited stronger GUS activity in roots than the T80 line and a weak activity in leaves with a root/leaf ratio similar to that of T80. This 2.1-kb promoter sequence possesses a high number of motifs previously described as root-specific or aspecific enhancers. However, this promoter-like sequence is not associated with a detectable transcript and its physiological significance is unclear.

Keywords: AbbreviationsGUS: β-Glucuronidase; Arabidopsis thaliana; HMG: High mobility protein; Key words Cryptic promoter; MAR: Matrix-associated region; Root-specific promoter; SAR: Scaffold-associated region.