PMab-213: A Monoclonal Antibody for Immunohistochemical Analysis Against Pig Podoplanin

Monoclon Antib Immunodiagn Immunother. 2019 Feb;38(1):18-24. doi: 10.1089/mab.2018.0048.

Abstract

Podoplanin (PDPN) is known to be expressed in normal tissues, including lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPN have already been established; however, mAbs against pig PDPN (pPDPN) are lacking. In the present study, mice were immunized with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and hybridomas producing mAbs against pPDPN were identified by flow cytometric screening. One of the mAbs, PMab-213 (IgG2b, kappa), could specifically detect CHO/pPDPN cells through flow cytometry and detect pPDPN through western blot analysis. KD of PMab-213 for CHO/pPDPN was determined to be 2.1 × 10-9 M, indicating a high affinity for CHO/pPDPN. Furthermore, PMab-213 strongly stained lymphatic endothelial cells, renal podocytes, and type I lung alveolar cells through immunohistochemistry. PMab-213 is expected to be useful in investigating the function of pPDPN.

Keywords: PDPN; PMab-213; pig podoplanin.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / genetics
  • Antibodies, Monoclonal / immunology*
  • CHO Cells
  • Cricetulus
  • Endothelial Cells / metabolism
  • Epitope Mapping
  • Epitopes / genetics
  • Epitopes / immunology
  • Flow Cytometry
  • Immunohistochemistry / methods
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology*
  • Mice
  • Podocytes / chemistry
  • Podocytes / metabolism*
  • Pulmonary Alveoli / cytology*
  • Pulmonary Alveoli / immunology
  • Swine / genetics

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Gp38 protein, mouse
  • Membrane Glycoproteins