Simultaneous detection of Dengue virus, Chikungunya virus, Zika virus, Yellow fever virus and West Nile virus

J Virol Methods. 2019 Jun:268:53-55. doi: 10.1016/j.jviromet.2019.03.014. Epub 2019 Mar 28.

Abstract

Although certain mosquito-borne virus, such as Dengue virus (DENV), Chikungunya virus (CHIKV), Zika virus (ZIKV), Yellow fever virus (YFV) and West Nile virus (WNV), are an important public health concern in those countries where transmitter mosquitoes are endemic, several cases of travelers from those endemic countries have been recently reported in Europe. Thus, early diagnosis of these viruses is essential for patient management and adoption of preventive measures. An assay for the simultaneous detection of DENV, CHIKV, ZIKV, YFV and WNV based on a multiplex real-time (RT)-PCR and its usefulness for diagnosis in infection screenings and surveillance of arbovirus in non-endemic countries are described.

Keywords: Arbovirus; Diagnosis; Multiplex PCR.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Chikungunya Fever / diagnosis
  • Chikungunya virus / isolation & purification*
  • Child
  • Child, Preschool
  • Dengue / diagnosis
  • Dengue Virus / isolation & purification*
  • Female
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Multiplex Polymerase Chain Reaction*
  • West Nile Fever / diagnosis
  • West Nile virus / isolation & purification*
  • Yellow Fever / diagnosis
  • Yellow fever virus / isolation & purification*
  • Young Adult
  • Zika Virus / isolation & purification*
  • Zika Virus Infection / diagnosis