Assessment of seven protocols to prepare Cutibacterium acnes bacterial lysates to measure its immunogenic potential and review of the literature

Anaerobe. 2019 Jun:57:75-81. doi: 10.1016/j.anaerobe.2019.03.019. Epub 2019 Mar 29.

Abstract

Seven protocols were tested to prepare cell wall extracts from live Cutibacterium acnes. Different parameters were modified: thawing/freezing and sonication/freezing cycles, to impact on mechanical degradation of the bacteria. Finally, the immunogenic potential of the extracts generated was evaluated by measuring IL-8 releases using an in vitro skin explants system. The aim of this article was to compare the existing protocols from the scientific literature, and also propose a standardized method developed in our facilities.

Keywords: Bacterial extract; Biomarkers; Cell wall membrane; Cutibacterium acnes; Inflammation; Innate immunity; Skin explants.

Publication types

  • Review

MeSH terms

  • Cell Extracts / immunology*
  • Cell Extracts / isolation & purification*
  • Cell Fractionation / methods
  • Cell Wall / immunology*
  • Humans
  • Immunity, Innate
  • Propionibacterium acnes / immunology*
  • Skin / immunology

Substances

  • Broncho-Vaxom
  • Cell Extracts