miRNA-365b promotes hepatocellular carcinoma cell migration and invasion by downregulating SGTB

Future Oncol. 2019 Jun;15(17):2019-2028. doi: 10.2217/fon-2018-0676. Epub 2019 Apr 3.

Abstract

Aim: miR-365b, a miRNA at chromosomal breakpoint, was often amplified and upregulated in human hepatocellular carcinoma (HCC). However, the role of miR-365b dysregulation remains unclear. Materials & methods: miR-365b function assays and its target gene analyses were performed. Results: We revealed that miR-365b promoted HCC cell motility and spreading. Furthermore, SGTB was found to be a downstream target of miR-365b, and knockdown of the SGTB gene could mimic the effect of miR-365b in hastening HCC cell migration and invasion. Conclusion: These results imply that miR-365b plays a tumor-promoting role in HCC by suppressing SGTB expression, offering novel potential targets for the treatment of HCC.

Keywords: HCC; invasion; miR-365b; migration.

MeSH terms

  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology
  • Carrier Proteins / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Down-Regulation
  • Epithelial-Mesenchymal Transition
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Humans
  • Liver / pathology
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / pathology
  • MicroRNAs / metabolism*
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism*
  • Neoplasm Invasiveness
  • RNA, Small Interfering / metabolism
  • Tissue Array Analysis

Substances

  • Carrier Proteins
  • MIRN365 microRNA, human
  • MicroRNAs
  • Molecular Chaperones
  • RNA, Small Interfering
  • SGTB protein, human