Long noncoding RNA SNHG12 promotes cell proliferation and activates Wnt/β-catenin signaling in prostate cancer through sponging microRNA-195

J Cell Biochem. 2019 Aug;120(8):13066-13075. doi: 10.1002/jcb.28578. Epub 2019 Apr 4.

Abstract

Long noncoding RNAs (lncRNAs) serve critical roles in multiple human malignant tumors, including prostate cancer (PCa). Currently, the biological role of oncogenic lncRNA SNHG12 in PCa remains largely unclear. In the present study, we found that SNHG12 was highly expressed in human PCa tissues and cell lines. In addition, gain-of-function and loss-of-function studies showed that overexpression of SNHG12 promoted, while downregulation suppressed the proliferation, invasion, and migration of PCa cells in vitro. Knockdown of SNHG12 also repressed PCa xenograft tumor growth in vivo. Further in-depth mechanistic studies showed that SNHG12 might serve as a competing endogenous RNA for miR-195 in PCa cells, and miR-195 expression level was negatively associated with the expression of SNHG12 in PCa tissues. Finally, we found that the activity of Wnt/β-catenin signaling is enhanced by SNHG12 overexpression and rescued by co-transfection with miR-195 mimics in PCa cells. Collectively, the present study indicated the oncogenic function of SNHG12 in PCa and our findings might provide a new target in the treatment of PCa.

Keywords: SNHG12; Wnt/β-catenin signaling; long noncoding RNA; microRNA-195; prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Animals
  • Base Sequence
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Mice, Nude
  • MicroRNAs / genetics*
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / therapy
  • RNA Interference
  • RNA, Long Noncoding / genetics*
  • RNAi Therapeutics / methods
  • Sequence Homology, Nucleic Acid
  • Tumor Burden / genetics
  • Wnt Signaling Pathway / genetics*
  • Xenograft Model Antitumor Assays / methods
  • beta Catenin / genetics*
  • beta Catenin / metabolism

Substances

  • MIRN195 microRNA, human
  • MicroRNAs
  • RNA, Long Noncoding
  • SNHG12 long non-coding RNA, human
  • beta Catenin