Detection of chloramphenicol in meat with a chemiluminescence resonance energy transfer platform based on molecularly imprinted graphene

Abstract

In this study, a novel composite was synthesized by polymerizing the dummy-template molecularly imprinted microspheres on the surface of magnetic graphene. This composite was used as recognition reagent and energy acceptor to develop a platform for determination of chloramphenicol according to the principle of chemiluminescence resonance energy transfer. The light signal was induced with luminolH₂O₂4-(imidazole-1-yl)phenol system, and the chemiluminescence intensity was positively correlated with the analyte concentration. The limit of detection for chloramphenicol in meat sample was 2.0 pg/g, and the recoveries from the standard fortified blank meat sample were in the range of 69.5%-97.3%. Furthermore, one single assay could be finished within 10 min, and the magnetic composite could be reused for at least thirty times. Therefore, this platform could be used as a rapid, simple, sensitive, accurate and recyclable tool for screening the residue of chloramphenicol in meat.

Keywords: Chemiluminescence resonance energy transfer; Chloramphenicol; Magnetic graphene; Meat; Molecularly imprinted microsphere.