Recombinant CV-A6 strains related to hand-foot-mouth disease and herpangina at primary care centers (Barcelona, Spain)

Future Microbiol. 2019 Apr:14:499-507. doi: 10.2217/fmb-2018-0336. Epub 2019 Apr 29.

Abstract

Aim: To describe the genetic diversity of enteroviruses (EV) causing hand, foot and mouth disease (HFMD) and herpangina, especially of coxsackievirus (CV)-A6, from patients attended at pediatric primary care centers during the 2017-2018 season. Methods: Phylogenetic analysis of partial VP1 region was performed for genetic characterization. The complete VP1 and 3Dpol proteins were sequenced for lineage determination and detection of recombination events. Results: An 80% of samples were EV laboratory-confirmed. CV-A6 was the most detected (70%) and associated with atypical HFMD (78%). The comparison of VP1 and 3Dpol phylogenies showed evidence of recombination in three strains, in which two shifted to CV-A16 3Dpol. Conclusion: The study provides recent information regarding the nonrecombinant and recombinant EVs related to HFMD at primary care centers.

Keywords: coxsackievirus A6; enteroviruses; hand, food and mouth disease; herpangina; pediatric infections.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / genetics
  • Child, Preschool
  • Disease Outbreaks
  • Enterovirus / classification
  • Enterovirus / genetics*
  • Enterovirus / isolation & purification
  • Enterovirus / pathogenicity*
  • Enterovirus Infections / epidemiology
  • Enterovirus Infections / virology
  • Female
  • Genotype
  • Hand, Foot and Mouth Disease / epidemiology
  • Hand, Foot and Mouth Disease / virology*
  • Herpangina / epidemiology
  • Herpangina / virology*
  • Humans
  • Infant
  • Male
  • Phylogeny
  • Primary Health Care*
  • Prospective Studies
  • Spain / epidemiology

Substances

  • Capsid Proteins
  • VP1 protein, Foot-and-mouth disease virus