Data on the negative regulation of invadopodia activity by MLCK

Rachel J Jerrell; Aron Parekh

doi:10.1016/j.dib.2019.103939

Data on the negative regulation of invadopodia activity by MLCK

Data Brief. 2019 Apr 24:24:103939. doi: 10.1016/j.dib.2019.103939. eCollection 2019 Jun.

Authors

Rachel J Jerrell¹, Aron Parekh^{1

2

3}

Affiliations

¹ Department of Otolaryngology, Vanderbilt University Medical Center, US.
² Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, US.
³ Department of Biomedical Engineering, Vanderbilt University, US.

Abstract

Actomyosin contractility can promote extracellular matrix (ECM) degradation by invadopodia in cancer cells. However, we previously found that inhibiting myosin light chain kinase (MLCK) with siRNA did not change force generation by the head and neck squamous cell carcinoma (HNSCC) cell line SCC-61. We provide data here that this targeted method of MLCK knockdown (KD) resulted in a significant increase in the amount of ECM degradation, number of actively degrading invadopodia, and the number of total invadopodia formed. These data are related to the research article entitled "Matrix rigidity differentially regulates invadopodia activity through ROCK1 and ROCK2" Jerrell and Parekh, 2016.

Keywords: Cancer; Contractility; Invadopodia; Invasion; MLCK.

Abstract

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