In Situ Vaccination of Tumors Using Plant Viral Nanoparticles

Abner A Murray; Mee Rie Sheen; Frank A Veliz; Steven N Fiering; Nicole F Steinmetz

doi:10.1007/978-1-4939-9516-5_10

In Situ Vaccination of Tumors Using Plant Viral Nanoparticles

Methods Mol Biol. 2019:2000:111-124. doi: 10.1007/978-1-4939-9516-5_10.

Authors

Abner A Murray¹, Mee Rie Sheen^{2

3

4}, Frank A Veliz⁵, Steven N Fiering^{2

3}, Nicole F Steinmetz^{6

7

8

9

10}

Affiliations

¹ Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
² Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth Lebanon, Norris Cotton Cancer Center, Lebanon, NH, USA.
³ Department of Genetics, Geisel School of Medicine at Dartmouth Lebanon, Norris Cotton Cancer Center, Lebanon, NH, USA.
⁴ Division of Hematology and Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
⁵ Department of Biomedical Engineering, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
⁶ Department of Biomedical Engineering, Case Western Reserve University School of Medicine, Cleveland, OH, USA. nfs11@case.edu.
⁷ Department of NanoEngineering, University of California-San Diego, La Jolla, CA, USA. nfs11@case.edu.
⁸ Department of Materials Science and Engineering, Case Western Reserve University School of Engineering, Cleveland, OH, USA. nfs11@case.edu.
⁹ Department of Macromolecular Science and Engineering, Case Western Reserve University School of Engineering, Cleveland, OH, USA. nfs11@case.edu.
¹⁰ Division of General Medical Sciences-Oncology, Case Western Reserve University, Cleveland, OH, USA. nfs11@case.edu.

Abstract

Viral nanoparticles are self-assembling units that are being developed and applied for a variety of applications. While most clinical uses involve animal viruses, a plant-derived virus, cowpea mosaic virus (CPMV) has been shown to have antitumor properties in mice when applied as in situ vaccine. Here we describe the production and characterization of CPMV and its use as in situ vaccines in the context of cancer. Subsequent analyses to obtain efficacy or mechanistic data are also detailed.

Keywords: B16F10; Cowpea mosaic virus (CPMV); Cytokine; Flow cytometry; Immunotherapy; In situ vaccine; Luminex multiplex; Melanoma.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cancer Vaccines*
Comovirus*
Immunotherapy / methods*
Melanoma / immunology
Melanoma / therapy
Mice
Nanoparticles*

Substances

Cancer Vaccines

Abstract

Publication types

MeSH terms

Substances

Grants and funding