EFL1 mutations impair eIF6 release to cause Shwachman-Diamond syndrome

Blood. 2019 Jul 18;134(3):277-290. doi: 10.1182/blood.2018893404. Epub 2019 May 31.

Abstract

Shwachman-Diamond syndrome (SDS) is a recessive disorder typified by bone marrow failure and predisposition to hematological malignancies. SDS is predominantly caused by deficiency of the allosteric regulator Shwachman-Bodian-Diamond syndrome that cooperates with elongation factor-like GTPase 1 (EFL1) to catalyze release of the ribosome antiassociation factor eIF6 and activate translation. Here, we report biallelic mutations in EFL1 in 3 unrelated individuals with clinical features of SDS. Cellular defects in these individuals include impaired ribosomal subunit joining and attenuated global protein translation as a consequence of defective eIF6 eviction. In mice, Efl1 deficiency recapitulates key aspects of the SDS phenotype. By identifying biallelic EFL1 mutations in SDS, we define this leukemia predisposition disorder as a ribosomopathy that is caused by corruption of a fundamental, conserved mechanism, which licenses entry of the large ribosomal subunit into translation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Animals
  • Cells, Cultured
  • DNA Mutational Analysis
  • Disease Models, Animal
  • Disease Susceptibility
  • Female
  • Genome-Wide Association Study
  • Humans
  • Infant
  • Male
  • Mice
  • Mice, Transgenic
  • Models, Molecular
  • Mutation*
  • Pedigree
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / genetics*
  • Peptide Elongation Factors / metabolism
  • Peptide Initiation Factors / biosynthesis*
  • Phenotype
  • Protein Conformation
  • Ribonucleoprotein, U5 Small Nuclear / chemistry
  • Ribonucleoprotein, U5 Small Nuclear / genetics*
  • Ribonucleoprotein, U5 Small Nuclear / metabolism
  • Shwachman-Diamond Syndrome / diagnosis
  • Shwachman-Diamond Syndrome / genetics*
  • Shwachman-Diamond Syndrome / metabolism*
  • Structure-Activity Relationship
  • Whole Genome Sequencing

Substances

  • EFL1 protein, human
  • Peptide Elongation Factors
  • Peptide Initiation Factors
  • Ribonucleoprotein, U5 Small Nuclear
  • eIF-6