MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A

Abstract

As a fundamental regulator of mitochondrial function, peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) acts as a powerful coactivator of many transcriptional factors that relate to steroidogenesis, while the regulatory mechanism remains unclear. In the present study, testosterone secretion of goat Leydig cells (LCs) mediated by miR-1197-3p via PPARGC1A was investigated. We found PPARGC1A protein was diversely localized in testis, and the expression of PPARGC1A in testis of 9-month-old goat was significantly higher than that in 3-month-old goat. In addition, suppression of PPARGC1A significantly decreased the testosterone secretion in goat LCs, as well as reduced the expressions of key steroidogenesis related genes [steroidogenic acute regulatory protein (StAR), cytochrome P450 family 11 subfamily A member 1 (CYP11A1), and 3 beta-hydroxysteroid dehydrogenase (3BHSD)], and overexpression of PPARGC1A showed the opposite effects. Moreover, we observed suppression of miR-1197-3p increased the synthesis of testosterone and promoted the expressions of PPARGC1A, StAR, CYP11A1, and 3BHSD by directly targeting PPARGC1A in the LCs. Furthermore, overexpression of PPARGC1A could alleviate miR-1197-3p induced aberrant steroidogenesis related gene expressions and testosterone synthesis. Taken together, miR-1197-3p could act as an essential regulator of LC testosterone secretion in goat testis by targeting PPARGC1A. These results provide a novel view of the regulatory mechanisms involved in male sexual maturation and help us to understand the molecular role of PPARGC1A in testosterone synthesis.

Keywords: Goat; Leydig cells; PPARGC1A; Testosterone; miR-1197-3p.