Input of recombinant phenotyping for the characterization of a novel acyclovir-resistance mutation identified in a patient with recurrent herpetic keratitis

Antiviral Res. 2019 Aug:168:183-186. doi: 10.1016/j.antiviral.2019.06.007. Epub 2019 Jun 11.

Abstract

We report here a case of an immunocompetent patient suffering from recurrent epithelial herpetic keratitis associated with the emergence of antiviral resistance. Indeed, the not previously described amino acid change L340R within herpes simplex virus thymidine kinase, was shown to confer acyclovir-resistance by recombinant phenotyping using bacmid technology.

Keywords: Antiviral resistance; Herpes simplex virus; Herpetic keratitis; Recombinant virus; Thymidine kinase.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology*
  • Acyclovir / therapeutic use
  • Adult
  • Antiviral Agents / pharmacology*
  • Antiviral Agents / therapeutic use
  • Drug Resistance, Viral / drug effects
  • Drug Resistance, Viral / genetics*
  • Herpesvirus 1, Human / drug effects
  • Herpesvirus 1, Human / genetics
  • Herpesvirus 1, Human / isolation & purification*
  • Humans
  • Keratitis, Herpetic / drug therapy
  • Keratitis, Herpetic / virology*
  • Male
  • Mutation
  • Phenotype
  • Recurrence
  • Thymidine Kinase / genetics

Substances

  • Antiviral Agents
  • Thymidine Kinase
  • Acyclovir