Detection of carbapenemase producers by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Ioannis K Neonakis; Demetrios A Spandidos

doi:10.1007/s10096-019-03620-0

Detection of carbapenemase producers by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Eur J Clin Microbiol Infect Dis. 2019 Oct;38(10):1795-1801. doi: 10.1007/s10096-019-03620-0. Epub 2019 Jun 28.

Authors

Ioannis K Neonakis¹, Demetrios A Spandidos²

Affiliations

¹ Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 71201, Heraklion, Greece. ineonakis@gmail.com.
² Laboratory of Clinical Virology, School of Medicine, University of Crete, 71003, Heraklion, Greece.

PMID: 31254128
DOI: 10.1007/s10096-019-03620-0

Abstract

Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently applied in detection of carbapenemase-producing Gram-negative isolates. In the present study, we review the latest developments in this field.

Keywords: 11,109 m/z peak; 28,544 m/z peak; Carbapenem resistance; Carbapenemase; Carbapenemase-producing Enterobacteriaceae; DOT-MGA; MALDI-TOF.

Publication types

Review

MeSH terms

Bacterial Proteins / analysis*
Gram-Negative Bacteria / enzymology*
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
beta-Lactamases / analysis*

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase