Transcriptome-wide analysis of immune responses in Eriocheir sinensis hemocytes after challenge with different microbial derivatives

Xingkun Jin; Weiwei Li; Minjie Xu; Youting Zhu; Yan Zhou; Qun Wang

doi:10.1016/j.dci.2019.103457

Transcriptome-wide analysis of immune responses in Eriocheir sinensis hemocytes after challenge with different microbial derivatives

Dev Comp Immunol. 2019 Dec:101:103457. doi: 10.1016/j.dci.2019.103457. Epub 2019 Jul 27.

Authors

Xingkun Jin¹, Weiwei Li², Minjie Xu³, Youting Zhu⁴, Yan Zhou⁵, Qun Wang⁶

Affiliations

¹ Institute of Marine Biology, School of Oceanography, Hohai University, Nanjing, 210098, China; Department of Biology, School of Life Science, East China Normal University, Shanghai, 200241, China. Electronic address: jinxingkun@hhu.edu.cn.
² Department of Biology, School of Life Science, East China Normal University, Shanghai, 200241, China. Electronic address: wwli@bio.ecnu.edu.cn.
³ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China. Electronic address: aseed@chgc.sh.cn.
⁴ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: ytzhu0820@gmail.com.
⁵ State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200433, China; Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China. Electronic address: zhouy@chgc.sh.cn.
⁶ Department of Biology, School of Life Science, East China Normal University, Shanghai, 200241, China. Electronic address: qwang@bio.ecnu.edu.cn.

PMID: 31362028
DOI: 10.1016/j.dci.2019.103457

Abstract

Knowledge about how Eriocheir sinensis interacts with microorganisms in its ambient environment is still lacking. Using RNA-Seq, we determined the most conserved genes and pathways compared with other animals and detected highly-induced immune genes in E. sinensis hemocytes post-in vivo challenge with different microbial derivatives. In total, 33.2 million high-quality reads were generated and assembled into 177,679 contigs. Completeness assessment and functional annotation were performed. Lipopolysaccharide, peptidoglycan, and β-1, 3-glucan stimulation induced 373, 173, and 108 differentially expressed (DE) transcripts, respectively. GO terms such as 'G-protein-coupled receptor binding', 'negative regulation of mitogen-activated protein kinase activity', and 'positive regulation of blood circulation' were enriched in the DE transcripts. Quantitative real-time PCR validated the data for selected genes. Our data contribute to understanding the immune defense mechanism in E. sinensis and the development of the innate immune system, thereby providing insights into disease control and prevention in aquaculture.

Keywords: Hemocytes; Innate immunity; Microbe-associated molecular pattern; Microbial derivative.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arthropod Proteins / genetics
Arthropod Proteins / immunology*
Brachyura / genetics
Brachyura / immunology*
Gene Expression Profiling
Hemocytes / immunology*
Immunity, Innate / genetics
Immunity, Innate / immunology*

Substances

Arthropod Proteins