Comparison of the cobas Human Immunodeficiency Virus 1 (HIV-1) Test Using the cobas 4800 System With COBAS AmpliPrep/COBAS TaqMan HIV-1 Test and Abbott RealTime HIV-1 Assay and Performance Evaluation of cobas HIV-1

Am J Clin Pathol. 2019 Oct 7;152(5):558-562. doi: 10.1093/ajcp/aqz075.

Abstract

Objectives: To compare quantified human immunodeficiency virus type 1 (HIV-1) viral load quantified using the cobas HIV-1 test with that obtained using the CAP/CTM HIV-1 and Abbott RealTime HIV-1 tests and evaluate the performance of cobas HIV-1 using the cobas 4800 system.

Methods: Clinical samples (n = 123) were quantitatively analyzed using the CAP/CTM HIV-1, Abbott RealTime HIV-1, and cobas HIV-1 tests, and the precision, linearity, and limit of detection of the cobas HIV-1 test were evaluated.

Results: Comparable results were obtained by both methods: ([log CAP/CTM HIV-1 value] = 0.979 * [log cobas HIV-1 test value] + 0.034) and ([log Abbott RealTime HIV-1 value] = 0.985 * [log cobas HIV-1 test value] + 0.027). cobas HIV-1 test results for 89.4% and 93.5% were within 0.5 log10 IU/mL of the CAP/CTM HIV-1 and Abbott RealTime HIV-1 results, respectively.

Conclusions: The cobas HIV-1 test showed good performance, and its results correlated well with those of other two tests.

Keywords: Correlation analysis; Human immunodeficiency virus; RNA quantification.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Anti-Retroviral Agents / therapeutic use
  • HIV Infections / blood
  • HIV Infections / drug therapy
  • HIV Infections / virology*
  • HIV-1 / genetics*
  • Humans
  • Limit of Detection
  • RNA, Viral / blood*
  • Real-Time Polymerase Chain Reaction / instrumentation
  • Real-Time Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Viral Load / instrumentation*
  • Viral Load / methods*

Substances

  • Anti-Retroviral Agents
  • RNA, Viral