Abstract
The use of induced pluripotent stem cells (iPSCs) as a cell source for producing cytotoxic T lymphocytes (CTLs) is expected to have advantages in the antigen specificity, rejuvenation profile, and reproducible number of CTLs. We have developed the way to differentiate CD8αβ T cells from TCR-transduced iPSCs (TCR-iPSCs). These T cells express monoclonal expression of the transduced TCR. Generating CD8αβ CTLs from TCR-iPSC could contribute to safe and effective allogeneic regenerative T cell immunotherapies.
Keywords:
T cell differentiation; TCR transduction; iPSCs.
MeSH terms
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Animals
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CD8 Antigens / immunology
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CD8 Antigens / metabolism
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Cell Culture Techniques / instrumentation
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Cell Culture Techniques / methods*
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Cell Differentiation
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Cell Line
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Cell Separation / instrumentation
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Cell Separation / methods
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Coculture Techniques / instrumentation
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Coculture Techniques / methods
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Culture Media / metabolism
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Cytokines / metabolism
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Flow Cytometry / instrumentation
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Flow Cytometry / methods
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Genetic Vectors / genetics
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Humans
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Immunotherapy, Adoptive / methods
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Induced Pluripotent Stem Cells / physiology*
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Lentivirus / genetics
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Mesenchymal Stem Cells
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Mice
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Neoplasms / immunology
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Neoplasms / therapy
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
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T-Lymphocytes, Cytotoxic / immunology
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T-Lymphocytes, Cytotoxic / metabolism
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T-Lymphocytes, Cytotoxic / transplantation*
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Transduction, Genetic / instrumentation
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Transduction, Genetic / methods*
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Transplantation, Homologous / methods
Substances
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CD8 Antigens
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CD8alphabeta antigen
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Culture Media
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Cytokines
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Receptors, Antigen, T-Cell
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Recombinant Proteins