C7 peptide inhibits hepatocellular carcinoma metastasis by targeting the HGF/c-Met signaling pathway

Cancer Biol Ther. 2019;20(12):1430-1442. doi: 10.1080/15384047.2019.1647051. Epub 2019 Aug 23.

Abstract

Hepatocellular carcinoma (HCC), characterized by a high rate of metastasis and recurrence after surgery, is caused by malignant proliferation of hepatocytes with epigenetic and/or genetic mutations. In particular, abnormal activation of the hepatocyte growth factor (HGF)-/c-mesenchymal-epithelial transition receptor (c-Met) axis is closely associated with HCC metastasis. Unfortunately, effective treatments or drugs that target the HGF/c-Met signaling pathway are still in the research pipeline. Here, a c-Met inhibitor named the C7 peptide, which can inhibit both HGF and c-Met, can significantly inhibit HGF-induced (but not EGF-induced) cell migration and suppress the phosphorylation of c-Met, Akt and Erk1/2. Moreover, the C7 peptide can also significantly suppress tumor metastasis in nude mice and the phosphorylation of c-Met. Together, our current findings, demonstrated that the C7 peptide can inhibit HGF-induced cancer cell migration and invasion through the inhibition of Akt and Erk1/2. Identification of a peptide that can block HGF/c-Met signaling provides new insight into the mechanism of HCC and future clinical treatments.

Keywords: C7 peptide; HGF/c-Met axis; hepatocellular carcinoma (HCC); invasion; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Line, Tumor
  • Cell Movement / drug effects*
  • Cell Proliferation / drug effects
  • Disease Models, Animal
  • Galanin / pharmacology*
  • Hepatocyte Growth Factor / metabolism*
  • Humans
  • Immunohistochemistry
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • Mice
  • Peptide Fragments / pharmacology*
  • Peptide Library
  • Protein Binding
  • Proto-Oncogene Proteins c-met / metabolism*
  • Signal Transduction / drug effects*
  • Substance P / analogs & derivatives*
  • Substance P / pharmacology
  • Xenograft Model Antitumor Assays

Substances

  • Peptide Fragments
  • Peptide Library
  • galanin (1-13)-spantide amide
  • Substance P
  • Hepatocyte Growth Factor
  • Galanin
  • Proto-Oncogene Proteins c-met

Grants and funding

This work was supported by Chinese National “973” Science Foundation, Grant number: [2002CB513100] and National Natural Sciences Foundation of China, Grant number [81772988].