Peptidomics analysis of umbilical cord blood reveals potential preclinical biomarkers for neonatal respiratory distress syndrome

Yin Hu; Juan Wang; Yahui Zhou; Hanying Xie; Xiangyun Yan; Xue Chu; Wenjuan Chen; Yiwen Liu; Xingyun Wang; Jun Wang; Aiqing Zhang; Shuping Han

doi:10.1016/j.lfs.2019.116737

Peptidomics analysis of umbilical cord blood reveals potential preclinical biomarkers for neonatal respiratory distress syndrome

Life Sci. 2019 Nov 1:236:116737. doi: 10.1016/j.lfs.2019.116737. Epub 2019 Sep 7.

Authors

Yin Hu¹, Juan Wang¹, Yahui Zhou¹, Hanying Xie², Xiangyun Yan¹, Xue Chu¹, Wenjuan Chen¹, Yiwen Liu², Xingyun Wang¹, Jun Wang³, Aiqing Zhang⁴, Shuping Han⁵

Affiliations

¹ Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China.
² Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China; The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.
³ Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China; The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China. Electronic address: 664586331@qq.com.
⁴ Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China; Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, 262 Zhongshan North Road, Nanjing, Jiangsu 210003, China. Electronic address: njaiqing@njmu.edu.cn.
⁵ Department of Pediatrics, The Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing 210004, China. Electronic address: shupinghan@njmu.edu.cn.

PMID: 31505194
DOI: 10.1016/j.lfs.2019.116737

Abstract

Aims: The purpose of this study was to investigate the pathophysiology and discover novel predictors of neonatal respiratory distress syndrome (NRDS) from a peptidomics perspective.

Main methods: Comparative profiling of umbilical cord blood from NRDS and control patients was performed by liquid chromatography tandem mass spectrometry technology. The underlying biological functions of the differentially expressed peptides (DEPs) were predicted by Gene Ontology (GO) and KEGG pathway analyses. The interactions of DEPs and their precursor proteins were explored by ingenuity pathway analysis (IPA). The sources and stability of DEPs were determined by online databases, including UniProt, SMART and ProtParam tool.

Key findings: A total of 251 DEPs were identified, of which 139 peptides were upregulated, and 112 peptides were downregulated (fold change ≥2.0, P < 0.05). These DEPs were predicted to be associated with respiratory failure, atelectasis, and morphogenesis of endothelial cells. These processes indicated that DEPs may play a role in NRDS. Among them, eleven stable DEPs might be used as preclinical biomarkers.

Significance: Our findings improve our understanding of NRDS and facilitate the discovery of candidate diagnostic biomarkers for NRDS from the perspective of peptidomics.

Keywords: Biomarkers; Clinical predictor; NRDS; Peptides; Preterm; Umbilical cord blood.

MeSH terms

Biomarkers / blood*
Case-Control Studies
Fetal Blood / metabolism*
Humans
Infant, Newborn
Peptide Fragments / blood*
Prognosis
Respiratory Distress Syndrome, Newborn / blood
Respiratory Distress Syndrome, Newborn / diagnosis*

Substances

Biomarkers
Peptide Fragments