Immune effector monocyte-neutrophil cooperation induced by the primary tumor prevents metastatic progression of breast cancer

Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21704-21714. doi: 10.1073/pnas.1907660116. Epub 2019 Oct 7.

Abstract

Metastatic behavior varies significantly among breast cancers. Mechanisms explaining why the majority of breast cancer patients never develop metastatic outgrowth are largely lacking but could underlie the development of novel immunotherapeutic target molecules. Here we show interplay between nonmetastatic primary breast cancer and innate immune response, acting together to control metastatic progression. The primary tumor systemically recruits IFNγ-producing immune effector monocytes to the lung. IFNγ up-regulates Tmem173/STING in neutrophils and enhances their killing capacity. The immune effector monocytes and tumoricidal neutrophils target disseminated tumor cells in the lungs, preventing metastatic outgrowth. Importantly, our findings could underlie the development of immunotherapeutic target molecules that augment the function of immune effector monocytes and neutrophils.

Keywords: CCL2; CCR2; STING; immune effector monocytes; metastatic breast cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cytotoxicity, Immunologic / immunology*
  • Female
  • Immunity, Innate / immunology
  • Immunotherapy / methods
  • Interferon-gamma / immunology
  • Mammary Neoplasms, Animal / pathology*
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NOD
  • Mice, SCID
  • Monocytes / immunology*
  • Neoplasm Metastasis / immunology
  • Neoplasm Metastasis / prevention & control
  • Neutrophils / immunology*
  • Tumor Microenvironment / immunology

Substances

  • IFNG protein, mouse
  • Membrane Proteins
  • Sting1 protein, mouse
  • Interferon-gamma