Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research

Erica C Pandolfi; Ernesto J Rojas; Enrique Sosa; Joanna J Gell; Timothy J Hunt; Sierra Goldsmith; Yuting Fan; Sherman J Silber; Amander T Clark

doi:10.1016/j.scr.2019.101576

Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research

Stem Cell Res. 2019 Oct:40:101576. doi: 10.1016/j.scr.2019.101576. Epub 2019 Sep 16.

Authors

Erica C Pandolfi¹, Ernesto J Rojas¹, Enrique Sosa¹, Joanna J Gell², Timothy J Hunt¹, Sierra Goldsmith³, Yuting Fan³, Sherman J Silber³, Amander T Clark⁴

Affiliations

¹ Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA.
² Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Department of Pediatrics, Division of Hematology-Oncology, David Geffen School of Medicine, Los Angeles, CA, USA.
³ Infertility Center of St Louis, St Luke's Hospital, St, Louis, MO, USA.
⁴ Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA. Electronic address: clarka@ucla.edu.

PMID: 31622877
DOI: 10.1016/j.scr.2019.101576

Abstract

We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDFs) (MZT04) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cells. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using teratomas and PluriTest. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility and infertility.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line / cytology*
Cell Line / metabolism
Cells, Cultured
Cellular Reprogramming
Female
Fibroblasts / cytology
Fibroblasts / metabolism
Homozygote
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Male
Middle Aged
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism

Substances

Octamer Transcription Factor-3
SOXB1 Transcription Factors