Lymphocyte Immunophenotyping and CD4/CD8 Ratio in Cerebrospinal Fluid for the Diagnosis of Sarcoidosis-related Uveitis

Ocul Immunol Inflamm. 2021 Feb 17;29(2):290-298. doi: 10.1080/09273948.2019.1678647. Epub 2019 Oct 31.

Abstract

Background: This study aimed to assess the diagnostic relevance of CD4/CD8 ratio in cerebrospinal fluid (CSF) for the etiological diagnosis work-up of uveitis.Methods: We consecutively included patients who were referred to our department for the diagnostic workup of intermediate and/or posterior uveitis. Etiological diagnoses were established in a blind manner regarding CD4/CD8 ratio.Results: Fifty-two patients were included. A diagnosis of ocular sarcoidosis was made in 15 (29%) patients, 21% had another determined diagnosis while 50% remained of undetermined origin. Median CD4/CD8 ratio in CSF was 4.57 (IQR 3.39-5.47) in ocular sarcoidosis, 1.74 (1.60-3.18) in uveitis due to other determined cause (P = .008), and 2.83 (2.34-3.54) in those with uveitis of undetermined origin (P = .007). CD4/CD8 ratio >3.23 was associated with a diagnosis of ocular sarcoidosis.Conclusion: Determination of CD4/CD8 ratio in CSF can be useful for diagnosis work-up since a CD4/CD8 ratio >3.23 in CSF is associated with ocular sarcoidosis.

Keywords: CD4/CD8 ratio; lymphocyte immunophenotyping; sarcoidosis; uveitis.

MeSH terms

  • Adult
  • Biomarkers / cerebrospinal fluid
  • CD4-CD8 Ratio
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • Female
  • Flow Cytometry
  • Humans
  • Immunophenotyping / methods*
  • Male
  • Middle Aged
  • Retrospective Studies
  • Sarcoidosis / cerebrospinal fluid
  • Sarcoidosis / complications
  • Sarcoidosis / immunology*
  • Uveitis / cerebrospinal fluid
  • Uveitis / diagnosis*
  • Uveitis / etiology

Substances

  • Biomarkers