Culture of putative Langerhans cell bone marrow precursors: characterization of their phenotype

Exp Hematol. 1988 Oct;16(9):764-8.

Abstract

We searched for the presence of human CD1-positive cells in bone marrow populations in order to characterize putative Langerhans cell precursors. Bone marrow progenitors were cultured in 0.8% methylcellulose supplemented with 10% granulocyte-macrophage (GM) colony-stimulating factor(s) GCT and HTB9. We compared the kinetics of these two factors and found that GCT was the more appropriate for our study. After 8 days of culture, colony-forming units of granulocyte-macrophages (CFU-GM) were tested for the presence of CD1-positive cells using the immunofluorescence technique. Positive cells were counted by cytofluorometric analysis: 9.4% CD1a (BL6), 13.4% CD1c (L161), 4.3% CD1b (NuT2), 4.6% CD2 (T11), and 25.5% CD33 (My9). Ultrastructural features and phenotype were then specified by the immunogold labeling technique using electron microscopy. A subpopulation of CD1-positive cells showed the ultrastructural morphology of bone marrow pro-monocyte/monocyte cells. By using well-characterized monoclonal antibodies, it was demonstrated that these cells expressed the following phenotype: CD14+, CD33+, CD4+, HLA-DR+, HLA-DP+, HLA-DQ-, OKT10-, CD2-. These data indicate that these bone marrow promonocyte/monocyte progenitors express a phenotype similar to that of epidermal Langerhans cells but the density of each antigen is much lower than that observed on mature skin dendritic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, Differentiation / analysis
  • Bone Marrow Cells*
  • Cells, Cultured
  • Colony-Forming Units Assay
  • Flow Cytometry
  • Humans
  • Langerhans Cells / classification
  • Langerhans Cells / cytology*
  • Langerhans Cells / ultrastructure
  • Phenotype
  • Stem Cells / classification
  • Stem Cells / cytology*
  • Stem Cells / ultrastructure

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation