Response surface method was used to optimize the ultrasonic assisted extraction of flavonoids from Crinum asiaticum

Miao Yu; Bing Wang; Zheng Qi; Guosong Xin; Wenlan Li

doi:10.1016/j.sjbs.2019.09.018

Response surface method was used to optimize the ultrasonic assisted extraction of flavonoids from Crinum asiaticum

Saudi J Biol Sci. 2019 Dec;26(8):2079-2084. doi: 10.1016/j.sjbs.2019.09.018. Epub 2019 Sep 14.

Authors

Miao Yu^{1

2}, Bing Wang³, Zheng Qi¹, Guosong Xin^{1

2}, Wenlan Li^{1

2}

Affiliations

¹ Research Center on Life Science and Environmental Science, Harbin University of Commerce, Harbin 150076, China.
² Postdoctoral Scientific Research Workstation, Harbin University of Commerce, Harbin 150076, China.
³ School of Food Engineering, Harbin University of Commerce, Harbin 150076, China.

Abstract

In this study, ultrasound-assisted extraction of flavonoid from Crinum asiaticum was studied through response surface methodology (RSM) to gain the best extraction process of flavonoid and enhance the extraction rate of flavonoid. In the following RSM experiment, we selected the corresponding data of every factor as the center point through the single-factor experiments, then the experimental data was subjected to multiple regression analysis. According to the statistical analysis results, the results were consistent with the polynomial regression model, the determination coefficient (R2) was 0.9769. The best conditions for maximum flavonoid yield were 60% ethanol concentration, 64 °C for extraction temperature, 1:28 (v/w) solid-to-liquid ratio with extraction time for 47 min. The best response of flavonoid yield was 1.63972%. The predicted results for best reaction conditions were in good agreement with experiment values. Ultrasound-assisted extraction method can enhance the extraction rate of flavonoid significantly. It is a powerful tool to extract of important phytochemicals from nature plant.

Keywords: Crinum asiaticum; Flavonoid; Response surface methodology; Ultrasound-assisted extraction.