A novel cell-penetrating Janus nanoprobe for ratiometric fluorescence detection of pH in living cells

Talanta. 2020 Mar 1:209:120436. doi: 10.1016/j.talanta.2019.120436. Epub 2019 Oct 11.

Abstract

pH regulates the function of many organelles and plays a pivotal role in requiring multitud cellular behaviors. Compared with single fluorescent probes, ratio fluorescent probes have higher sensitivity and immunity to interference. Herein, a novel Janus ratio nanoprobe was developed for intracellular pH detection. Modified rhodamine B probe and fluorescein isothiocyanate (FITC) were individually encapsulated in the independent hemispheres of Janus microparticles fabricated via Pickering emulsion. Moreover, it exhibits a satasified ratiometric detection of pH compared to the previous core-shell structure and organic small molecule probe. Accordingly, the Janus nanoprobe possesses many important features as an attractive sensor, including high anti-jamming capability, excellent stability, good reversibility and low cytotoxicity. Variations of the two fluorescence intensities (Fgreen/Fred) resulted in a ratiometric pH fluorescent sensor, which can respond to wide range of pH values from 3 to 8. To be more specific, with a single excitation wavelength of 488 nm, there are dual emission bands centered at 538 nm and 590 nm. Also the Janus nanoprobe displays a excellent linear relationship in the physiologically relevant pH range of 4.0-6.0. Consequently, detecting of pH and imaging was successfully achieved in living cells, which provides a simple and reliable method for detecting intracelluar pH and other similar substances.

Keywords: Intracelluar pH; Janus nanoprobe; Ratiometric detection.

MeSH terms

  • Cell Line, Tumor
  • Cell Survival
  • Fluorescein-5-isothiocyanate / chemistry*
  • Fluorescein-5-isothiocyanate / toxicity
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / toxicity
  • Humans
  • Hydrogen-Ion Concentration
  • Microscopy, Fluorescence
  • Nanoparticles / chemistry*
  • Nanoparticles / toxicity
  • Rhodamines / chemical synthesis
  • Rhodamines / chemistry*
  • Rhodamines / toxicity
  • Silicon Dioxide / chemistry
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Rhodamines
  • Silicon Dioxide
  • Fluorescein-5-isothiocyanate
  • rhodamine B